|
| Status |
Public on Nov 04, 2024 |
| Title |
Y27_K975_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
mesothelioma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: mesothelioma
cell line: Y-MESO-27
treatment: K-975
|
| Treatment protocol |
Cells were cultured in the presence or absence of 1 μM K-975 for 48 hours
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was isolated using the RNeasy Mini Kit (Qiagen) and processed as per manufacturer’s instructions.
Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina and the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
Sample 22
|
| Data processing |
The fastq files were analyzed using the RaNA-seq tool
Assembly: Human, Homo sapiens (GRCh38)
Supplementary files format and content: csv file includes raw counts for each experiments
|
| |
|
| Submission date |
Mar 29, 2024 |
| Last update date |
Nov 04, 2024 |
| Contact name |
Tatsuhiro Sato |
| E-mail(s) |
[email protected]
|
| Organization name |
Aichi Cancer Center Research Institute
|
| Department |
Division of Cancer Biology
|
| Street address |
1-1 Kanokoden
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
4648681 |
| Country |
Japan |
| |
|
| Platform ID |
GPL20795 |
| Series (1) |
| GSE262853 |
Gene expression analysis in mesothelioma cell lines with and without K-975 treatment |
|
| Relations |
| BioSample |
SAMN40658926 |
| SRA |
SRX24105939 |
