|
| Status |
Public on Dec 01, 2011 |
| Title |
PU.1 ki/ki rep3 |
| Sample type |
RNA |
| |
|
| Source name |
sorted HSCs (lin-sca1+ckit+CD150+CD48-) of PU.1ki/ki mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: sorted HSCs (lin-sca1+ckit+CD150+CD48-)
genotype/variation: knock-in mice
age: adult
|
| Treatment protocol |
Adult HSCs were collected after sorting using the markers (lin-sca1+ckit+CD150+CD48-) and placed on ice in the Trizol solution (GibcoBRL).
|
| Growth protocol |
All mice were kept in a sterile barrier facility approved by the Beth Israel Deaconess Medical Center Institutional Animal Care and Use Committee.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
|
| Data processing |
The data were analyzed using RMA in the bioconducter and normalized using cross correlation. The trimmed mean target intensity of each array was set to 10.
|
| |
|
| Submission date |
Oct 18, 2011 |
| Last update date |
Dec 01, 2011 |
| Contact name |
Henry Yang |
| E-mail(s) |
[email protected]
|
| Organization name |
Cancer Science Institute of Singapore
|
| Street address |
28 Medical Drive
|
| City |
Singapore |
| ZIP/Postal code |
117456 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE33031 |
PU.1 restricts adult hematopoietic stem cell proliferation via cell specific autoregulation |
|
