|
| Status |
Public on Feb 25, 2021 |
| Title |
Nanog_R_day0_+Dox_rep2 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Embryonic Stem Cell
|
| Organism |
Mus musculus |
| Characteristics |
rescue clone: Nanog_R
enzyme: MspI
agent: doxycycline
day: 0
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA extracted with Gentra Puregene Cell Kit (Qiagen) and digested with the isoschizomer enzymes HpaII or MspI (NEB, Ipswich, MA).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed with the NimbleGen Dual-color DNA labeling kit by the Weill Cornell Medical College Epigenomics Core Facility using NimbleGen Systems Inc., Madison, WI USA, standard operating protocol. See www.nimblegen.com.
|
| |
|
| Channel 2 |
| Source name |
Embryonic Stem Cell
|
| Organism |
Mus musculus |
| Characteristics |
rescue clone: Nanog_R
enzyme: HpaI
agent: doxycycline
day: 0
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA extracted with Gentra Puregene Cell Kit (Qiagen) and digested with the isoschizomer enzymes HpaII or MspI (NEB, Ipswich, MA).
|
| Label |
Cy5
|
| Label protocol |
Labeling was performed with the NimbleGen Dual-color DNA labeling kit by the Weill Cornell Medical College Epigenomics Core Facility using NimbleGen Systems Inc., Madison, WI USA, standard operating protocol. See www.nimblegen.com.
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed by the Weill Cornell Medical College Epigenomics Core Facility using NimbleGen Systems Inc., Madison, WI USA, standard operating protocol. See www.nimblegen.com. Section Two Color Sample Labeling.
|
| Scan protocol |
Scanning was performed with NimbleGen MS 200 by the Weill Cornell Medical College Epigenomics Core Facility using NimbleGen Systems Inc., Madison, WI USA, standard operating protocol. See www.nimblegen.com.
|
| Description |
Nanog_R_day0_+Dox_rep2: This sample is Nanog_rescue clone at Day0 in the presence of Doxycycline replicate 2 of the three replicates.
|
| Data processing |
The raw data (.pair file) was processed as described in Thompson et al,(2008)
|
| |
|
| Submission date |
Oct 24, 2011 |
| Last update date |
Feb 25, 2021 |
| Contact name |
Ana Sevilla |
| E-mail(s) |
[email protected]
|
| Phone |
+34 675348398
|
| Organization name |
Universidad de Barcelona
|
| Department |
Departamento de Biologia Celular, Fisiologia e Inmunologia
|
| Lab |
4 Floor.
|
| Street address |
Avda Diagonal 643. Edificio Malagef 4rd Floor, Faculty of Biology
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL14781 |
| Series (2) |
| GSE31842 |
An Esrrb and Nanog Cell Fate Regulatory Module Controlled by Feed Forward Loop Interactions. |
| GSE33191 |
An Esrrb and Nanog Cell Fate Regulatory Module Controlled by Feedback Interactions [methylation] |
|
