|
| Status |
Public on Jan 04, 2012 |
| Title |
TßRII-Het Bugle set #2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse back skins
|
| Organism |
Mus musculus |
| Characteristics |
gender: Female
age: P72-74
strain: mixed C57BL/6 and CD1
genotype/variation: TßRII-Het
sorting markers: Rosa26-YFP, P-cadherin, CD34
cell type: activated bulge HFSCs
|
| Treatment protocol |
No treatment
|
| Growth protocol |
HG and bulge HFSCs and Rosa26YFP+ total skin keratinocytes were FACS-purifed from the back skins of indicated ages of mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G Scanner.
|
| Description |
Gene expression data from activated bulge HFSCs
|
| Data processing |
The data were analyzed with MAS5.0 method using Gene Pattern analysis settings and median scaling as normalization method.
|
| |
|
| Submission date |
Nov 03, 2011 |
| Last update date |
Jan 04, 2012 |
| Contact name |
Naoki Oshimori |
| E-mail(s) |
[email protected]
|
| Organization name |
The Rockefeller University/HHMI
|
| Lab |
Laboratory of Mammalian Cell Biology and Development
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE33471 |
Paracrine TGFß Signaling Counterbalances BMP-Mediated Repression in Hair Follicle Stem Cell Activation |
|
