|
| Status |
Public on Oct 20, 2012 |
| Title |
twin pair 6_lower IQ |
| Sample type |
RNA |
| |
|
| Source name |
twin 6A_Lower_IQ
|
| Organism |
Homo sapiens |
| Characteristics |
twin pair id: 6
relative iq within twin pair: Lower
cell type: B-lymphoblastoid cell
|
| Growth protocol |
B-lymphoblastoid cell lines were grown in a RPMI-1640 (WAKO) medium and supplemented with 10% FBS (Gibco) and antibiotics (Wako).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated using RNeasy Plus Mini Kit (Qiagen)
|
| Label |
Tdt
|
| Label protocol |
300ng of total RNA was processed using Ambion WT expression Kit (Applied Biosystems) and the Affymetrix GeneChip WT Terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 17+/-1 hr at 45C on Human Genechip 1.0st arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400. GeneChip Hybridization, Wash, and Stain Kit (Affymetrix) was used.
|
| Scan protocol |
Gene chips were scanned using Genechip Scanner 3000.
|
| Description |
twin 6A
expression profile from Twin Pair ID6, lower IQ twin
|
| Data processing |
The data were analysed using Partek Genomic Suite 6.5 (Agilent). We normalized the data according to the RMA algorithm.
|
| |
|
| Submission date |
Nov 04, 2011 |
| Last update date |
Oct 20, 2012 |
| Contact name |
Chihchieh Yu |
| E-mail(s) |
[email protected]
|
| Organization name |
Kobe University
|
| Street address |
7-5-1 kusunoki-cho, chuo-ku
|
| City |
Kobe-shi |
| ZIP/Postal code |
6500017 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE33476 |
Expression data from phenotypically discordant monozygotic twin lymphoblasts |
| GSE33478 |
Genome-wide DNA methylation and gene expression analyses of monozygotic twin discordant for intelligence levels |
|
