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Sample GSM8305942 Query DataSets for GSM8305942
Status Public on Jun 06, 2024
Title Culex adults, mock, WNV, 24 h, biol rep 8
Sample type SRA
 
Source name Whole animal
Organism Culex quinquefasciatus
Characteristics tissue: Whole animal
genotype: WT
treatment: 0 pM insulin, WNV infection
Treatment protocol 6-9 day old adult female mosquitoes were fed on defibrinated chicken blood experimental glass feeders with 1e7 PFU WNV-Kunjin +/- 170 pM bovine insulin. Mosquitoes were deprived of sucrose 48 hours prior to experimental feedings.
Growth protocol Adult female mosquitoes were fed on defibrinated chicken blood in hog sausage casing. Adults were provided continuous access to sucrose.
Extracted molecule total RNA
Extraction protocol Animals were homogenized in Trizol reagent. RNA was harvested using Trizol reagent and Direct-Zol RNA extraction kit (Zymo Research). 2.5 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina) following manufacturer's protocols.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing CLC Genomics Workbench v 11.0.1
Sequence reads were imported using Illumina paired importer (FASTQ).
Trimmed sequence reads were mapped to GCA_000209185.1 using CLC genomic benchwork (parameters: Discard read names=No, Discard quality scores=No, Paired orientation=Paired reads (forward-reverse), Minimum distance=200, Maximum distance=300, Quality score=NCBI/Sanger or Illumina Pipeline 1.8 and later, Remove failed reads=Yes, MiSeq de-multiplexing=No).
Read count extraction and normalization were performed using CLC genomic benchwork.
Assembly: GCA_000209185.1
Supplementary files format and content: tab-delimited text file of raw gene expression data for all samples
 
Submission date Jun 04, 2024
Last update date Jun 06, 2024
Contact name Alan G. Goodman
Organization name Washington State University
Department School of Molecular Biosciences
Street address 100 Dairy Rd., BLS 202
City Pullman
State/province WASHINGTON
ZIP/Postal code 99164
Country USA
 
Platform ID GPL34558
Series (1)
GSE269077 Effect of insulin on West Nile virus (WNV) infection in Culex quinquefasciatus
Relations
BioSample SAMN41675172
SRA SRX24801723

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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