|
| Status |
Public on Jun 06, 2024 |
| Title |
Culex adults, insulin, WNV, 24 h, biol rep 4 |
| Sample type |
SRA |
| |
|
| Source name |
Whole animal
|
| Organism |
Culex quinquefasciatus |
| Characteristics |
tissue: Whole animal
genotype: WT
treatment: 170 pM insulin, WNV infection
|
| Treatment protocol |
6-9 day old adult female mosquitoes were fed on defibrinated chicken blood experimental glass feeders with 1e7 PFU WNV-Kunjin +/- 170 pM bovine insulin. Mosquitoes were deprived of sucrose 48 hours prior to experimental feedings.
|
| Growth protocol |
Adult female mosquitoes were fed on defibrinated chicken blood in hog sausage casing. Adults were provided continuous access to sucrose.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Animals were homogenized in Trizol reagent. RNA was harvested using Trizol reagent and Direct-Zol RNA extraction kit (Zymo Research). 2.5 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina) following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
CLC Genomics Workbench v 11.0.1
Sequence reads were imported using Illumina paired importer (FASTQ).
Trimmed sequence reads were mapped to GCA_000209185.1 using CLC genomic benchwork (parameters: Discard read names=No, Discard quality scores=No, Paired orientation=Paired reads (forward-reverse), Minimum distance=200, Maximum distance=300, Quality score=NCBI/Sanger or Illumina Pipeline 1.8 and later, Remove failed reads=Yes, MiSeq de-multiplexing=No).
Read count extraction and normalization were performed using CLC genomic benchwork.
Assembly: GCA_000209185.1
Supplementary files format and content: tab-delimited text file of raw gene expression data for all samples
|
| |
|
| Submission date |
Jun 04, 2024 |
| Last update date |
Jun 06, 2024 |
| Contact name |
Alan G. Goodman |
| Organization name |
Washington State University
|
| Department |
School of Molecular Biosciences
|
| Street address |
100 Dairy Rd., BLS 202
|
| City |
Pullman |
| State/province |
WASHINGTON |
| ZIP/Postal code |
99164 |
| Country |
USA |
| |
|
| Platform ID |
GPL34558 |
| Series (1) |
| GSE269077 |
Effect of insulin on West Nile virus (WNV) infection in Culex quinquefasciatus |
|
| Relations |
| BioSample |
SAMN41675168 |
| SRA |
SRX24801727 |
