|
| Status |
Public on Nov 19, 2024 |
| Title |
Fat bodies, Control, rep4 |
| Sample type |
SRA |
| |
|
| Source name |
Fat bodies
|
| Organism |
Hermetia illucens |
| Characteristics |
tissue: Fat bodies
agent: Control
|
| Treatment protocol |
Eeach replicate comprising 50 larvae in 25 grams of standard Gainesville diet. The treatments were administered as follows: 1) Control (no fungi added) - 50 ml sterile water. 2) B. bassiana - commercial strain GHA-11.3% (RIMI). Fungal spore quantification was performed using a hemocytometer, and a concentration of 107 cells/ml was selected (10 ml of commercial strain and 40 ml of water) as previously described 16,17. 3) C. tropicalis-1 gram of yeast pellet in 50ml sterile water. 4) S. cerevisiae -1 gram of yeast pellet in 50ml sterile water.
|
| Growth protocol |
The larvae were reared on a sterile standard Gainesville diet for 10 days in rearing chamber (27 ± 1.0 °C, RH 70% ± 5%, and D/L 12/12 photoperiod) and then transferred into four treatment groups.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Four days after fungal treatment 5 larvae from each biological replication were dissected and the fat body was collected into Eppendorf on dry ice. RNA was then extracted using RNA extraction kit (Hybrid-RTM-GeneAll) and DNase removal performed using Clean-Up RNA concentrator Kit (A&A BIOTECHNOLOGY).
The libraries were then constructed using ++NEBNext Ultra II RNA Library Prep Kit and NEBNext Poly(A) mRNA Magnetic Isolation Module
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq X |
| |
|
| Description |
conrol4
|
| Data processing |
To analyze the RNA sequencing data, we used the nf-core/rnaseq bioinformatics pipeline, version 3.14.025. The STAR–Salmon route was chosen for alignment and quantification.
Assembly: GCF_905115235.1_ _iHerIll2.2.curated.20191125.
Supplementary files format and content: csv file includes raw counts for each sample
|
| |
|
| Submission date |
Jul 14, 2024 |
| Last update date |
Nov 19, 2024 |
| Contact name |
Neta Herman |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
Hebrew University Of Jerusalem, Migal
|
| Department |
Gentics, Nutrition
|
| Lab |
Shagiv Shifman; Opatovsky Itai
|
| Street address |
Kiryat Shemona
|
| City |
Jerusalem, Kiryat Shemona |
| ZIP/Postal code |
1101202 |
| Country |
Israel |
| |
|
| Platform ID |
GPL34702 |
| Series (1) |
| GSE272202 |
Regulation of Antimicrobial Peptides in Hermetia illucens in Response to Fungal Exposure |
|
| Relations |
| BioSample |
SAMN42486837 |
| SRA |
SRX25321940 |
