|
| Status |
Public on Aug 31, 2012 |
| Title |
DeltaPT d4 b |
| Sample type |
RNA |
| |
|
| Source name |
Mouse whole lung
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
gender: female
age: 7 wk
tissue: lung
infection: 1 x 10^6 CFU DeltaPT Bordetella pertussis
time: 4 dpi
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RNA STAT-60 reagent (Tel Test, Inc.), followed by DNase I treatment and clean-up with QIAGEN RNeasy mini kit in accordance with the protocol provided with the kit. Quality control was performed with an Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit from Ambion.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
deltaPT-treated, sacrificed 4 dpi, biological replicate 2
|
| Data processing |
The data were normalized by median method implemented in R package beadarray.
|
| |
|
| Submission date |
Nov 29, 2011 |
| Last update date |
Aug 31, 2012 |
| Contact name |
Nicholas Carbonetti |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Maryland Baltimore
|
| Department |
Microbiology and Immunology
|
| Lab |
Carbonetti Lab
|
| Street address |
685 W. Baltimore St. HSF I-380
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE33995 |
The effect of pertussis toxin on lung airway gene expression during B. pertussis infection in mice |
|
