|
| Status |
Public on Dec 18, 2011 |
| Title |
4hr_C57_GSS_101_lung |
| Sample type |
RNA |
| |
|
| Source name |
lung
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
exposure: GMA-SS
recovery time: 4h
|
| Treatment protocol |
Mice were exposed to either air or gas metal arc-stainless steel welding fume for 10 days (3 hours/day) at 40 mg/m3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Lung RNA isolated from whole lung homogenates using the TRIzol method and cleaned with a Rneasy Mini Kit. Aorta was homogenized with a Qiagen Tissue Lyser and RNA was isolated using the Rneasy fibrous mini kit. Whole blood RNA was isolated using the Mouse RiboPure Blood RNA Isolation Kit and globin RNA was removed using the GLOBINclear kit.
|
| Label |
biotin
|
| Label protocol |
Illumina TotalPrep RNA Amplification Kit (Applied Biosystems Inc., Foster City, CA, Catalog #AMIL1791)
|
| |
|
| Hybridization protocol |
20 hour hybridization period at 58°C
|
| Scan protocol |
Illumina BeadStation 500G - BeadArray Reader standard
|
| Description |
4320221051_F
replicate 2
|
| Data processing |
Force positive background correction, variance stabilizing transformation, robust spline normalization
|
| |
|
| Submission date |
Nov 30, 2011 |
| Last update date |
Dec 18, 2011 |
| Contact name |
Patti Christine Zeidler-Erdely |
| E-mail(s) |
[email protected]
|
| Phone |
3042855881
|
| Organization name |
CDC/NIOSH
|
| Department |
HELD
|
| Street address |
1095 Willowdale Road
|
| City |
Morgantown |
| State/province |
WV |
| ZIP/Postal code |
26505 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE34056 |
Type 1 interferon and pattern recognition receptor signaling following particulate matter inhalation |
|
