|
| Status |
Public on Jul 25, 2012 |
| Title |
testis-wt-4 |
| Sample type |
RNA |
| |
|
| Source name |
testis day P23
|
| Organism |
Mus musculus |
| Characteristics |
tissue: testis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
cRNA was hybridized to the GeneChip Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station Fluidics 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray scanner 3000
|
| Description |
Gene expression data from wildtype testis
|
| Data processing |
The data was normalized by Robust Multi-Array average (RMA) algorithm
|
| |
|
| Submission date |
Dec 02, 2011 |
| Last update date |
Jul 25, 2012 |
| Contact name |
frank sleutels |
| Organization name |
ErasmusMC
|
| Department |
cell biology and genetics
|
| Street address |
Dr Molewaterplein 50
|
| City |
Rotterdam |
| ZIP/Postal code |
3015 GE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE34091 |
Nucleosome dynamics specifies genome-wide binding of the male germ cell gene regulator CTCFL and of CTCF [Mouse430_2 Expression] |
| GSE34093 |
Nucleosome dynamics specifies genome-wide binding of the male germ cell gene regulator CTCFL and of CTCF |
|
