|
| Status |
Public on Dec 06, 2011 |
| Title |
Met28_5M |
| Sample type |
genomic |
| |
|
| Source name |
genomic DNA from metastatic prostate cancer
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
tissue: metastatic prostate cancer
tissue type: Soft Tissue Metastasis
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Tumors were removed within 2 hours of death in a rapid autopsy. A single pathologist sectioned 15 tissue slices at 15-um from areas with extensive tumor involvement. DNA was extracted with a Wizard Genomic DNA Isolation kit (Promega) and purified by phenol/chloroform extraction. All DNAs were of sufficient quality and quantity for genomic analysis by gel chromatography and UV-Vis spectrophotometery.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
DNA was bisulfite-converted using the EZ DNA Methylation Kit (Zymo) then processed per standard Illumina protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
metastatic prostate cancer
|
| Data processing |
BeadStudio software v3.2. Average Beta and Unmethylated and methylated signal intensities are provided.
|
| |
|
| Submission date |
Dec 05, 2011 |
| Last update date |
Dec 06, 2011 |
| Contact name |
Terence Friedlander |
| E-mail(s) |
[email protected]
|
| Phone |
415-514-2559
|
| Organization name |
UCSF
|
| Department |
Hemotology-Oncology
|
| Lab |
Paris Lab
|
| Street address |
1450 3rd Street
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL8490 |
| Series (1) |
| GSE34174 |
Whole Genome Methylation Analysis of Metastatic Castration Resistant Prostate Cancer |
|
