|
| Status |
Public on Mar 01, 2012 |
| Title |
CCALD patient fibroblast, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
dermal fibroblast from CCALD patient
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: dermal fibroblast
genotype: CCALD patient
|
| Treatment protocol |
Fibroblasts are dissociated with 0.05% trypsin at 37°C, 5% CO2 for 5minutes and collected by centrifugation; iPSCs are dissociated with 1mg/ml collagenase IV at 37°C, 5% CO2 for 5minutes, manually detached and collected by centrifugation
|
| Growth protocol |
Fibroblasts and iPSCs are cultured in 1:1 ratio of DMEM/F12 medium supplemented with 20% KSR at 37°C, 5% CO2 until confluence
|
| Extracted molecule |
total RNA |
| Extraction protocol |
as recommended by affymetrix
|
| Label |
biotin
|
| Label protocol |
followed affymetrix protocol
|
| |
|
| Hybridization protocol |
followed affymetrix protocol
|
| Scan protocol |
followed affymetrix protocol
|
| Description |
Gene expression data of CCALD patient dermal fibroblast
|
| Data processing |
WebArray software was used to generate log2-transformed gene expression values using the RMA algorithm
|
| |
|
| Submission date |
Dec 09, 2011 |
| Last update date |
Mar 01, 2012 |
| Contact name |
Joseph Gerard Hacia |
| E-mail(s) |
[email protected]
|
| Phone |
323-442-3030
|
| Organization name |
University of Southern California
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Hacia Lab
|
| Street address |
2250 Alcazar Street, IGM 261
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90089 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE34309 |
Gene expression profiles of fibroblasts and fibroblast-reprogrammed induced pluripotent stem cells (iPSCs) from childhood cerebral adrenoleukodystrophy patients and healthy controls |
|
