|
| Status |
Public on Dec 10, 2011 |
| Title |
dex24hr_2 |
| Sample type |
RNA |
| |
|
| Source name |
cultured human airway smooth muscle, 24 hour dex treatment, replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: no history of asthma
tissue: smooth muscle cells
treatment: dexamethasone for 24 hr
|
| Treatment protocol |
Cells were treated for 4 hours or 24 hours with 1 micromnolar dexamethasone dissolved in ethanol
|
| Growth protocol |
Primary cultured human airway smoothmuscle were grown in Hams/F12 with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol frolloed by purification with Invitrogne midilink columns
|
| Label |
Cy3
|
| Label protocol |
RNA was labeled with Cy3-CTP using the Agilent low RNA input fluorescent linear amplification kits following the manufacturers protocol
|
| |
|
| Hybridization protocol |
Labeled cRNA was assessed using the Nandrop ND-100 (Nanodrop Technologies, Inc., Wilmington DE), and equal amounts of Cy3 labeled target were hybridized to Agilent whole mouse genome 4x44K Ink-jet arrays for 14 hours
|
| Scan protocol |
Arrays were scanned using the Agilent microarray scanner
|
| Description |
human airway smooth muscle gene expression, 24 hours dexamethasone
|
| Data processing |
raw signal intensities were extracted with Feature Extraction v9.1 software
|
| |
|
| Submission date |
Dec 09, 2011 |
| Last update date |
Dec 10, 2011 |
| Contact name |
Anthony Gerber |
| E-mail(s) |
[email protected]
|
| Phone |
3032702783
|
| Organization name |
National Jewish Health
|
| Department |
Medicine
|
| Street address |
1400 Jackson St
|
| City |
Denver |
| State/province |
CO |
| ZIP/Postal code |
80238 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE34313 |
Airway smooth muscle reponse to dexamathasone at 4 and 24 hours |
|
