|
| Status |
Public on Sep 02, 2024 |
| Title |
Clone B P30 +GLN |
| Sample type |
SRA |
| |
|
| Source name |
CHO K1GS
|
| Organism |
Cricetulus griseus |
| Characteristics |
cell line: CHO K1GS
cell type: Chinese Hamster Ovary
genotype: Clone B
treatment: 6mM L-Glutamine
|
| Treatment protocol |
The cells were passaged every 3 days with viable cell density (VCD) and viability was determined by hemocytometer using trypan blue dye exclusion. The cells were then seeded in fresh media at 0.3 x 106 cells/mL. Cells were passaged for a total of 30 passages over 90 days during the aging process.
|
| Growth protocol |
These cells were cultured with imMEDIAte ADVANTAGE 87093C (SAFC) media, a serum-free custom made medium by Milipore Sigma, in 125 mL shake flasks (Fisher Scientific) with a working volume of 30mL. Incubation was done in a humidified orbital shaking incubator set at 37C, 80% humidity, 5% CO2 and 125 rpm shaking. The two clonal cell lines were thawed and seeded at 0.3 x 106 cells/mL.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Chromium Single Cell 3’ library construction kit
Chromium Single Cell 3’ library construction kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10X Genomics
|
| Data processing |
The demultiplexing, barcoded processing, gene counting, and aggregation were made using Cell Ranger version 6.0.2
Assembly: CHOK1GS_HD (GCA_900186095.1)
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Aug 28, 2024 |
| Last update date |
Sep 02, 2024 |
| Contact name |
Luke B Morina |
| Organization name |
Johns Hopkins University
|
| Street address |
3400 N Charles St; CLARK HALL Rm118a
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21218 |
| Country |
USA |
| |
|
| Platform ID |
GPL27425 |
| Series (1) |
| GSE275930 |
Investigating Subpopulation Dynamics in Clonal CHO-K1 Cells with Single-Cell RNA Sequencing |
|
