|
| Status |
Public on Feb 01, 2013 |
| Title |
PBLs of patient 13 pre therapy |
| Sample type |
RNA |
| |
|
| Source name |
periphereal blood lymphocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PBLs from leucapheresis product
disease state: metastatic renal cell carcinoma
group: pre therapy
|
| Treatment protocol |
immunotherapy consisted of intranodal vaccination of dendritic cells loaded with tumor renal cell carcinoma lysate, intravenous infusion of Interleukin 2 and subcutaneous injections of Interferon-alpha2a
|
| Growth protocol |
PBLs were isolated from pheresis product by elutrition fractionation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated using RNAeasy kit (Quiagen) according to manufacteurer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 5,5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
cDNA was hybridized to the GeneChip Human Gene 1.0 ST Array according to Affymetrix guidelines
|
| Scan protocol |
the stained array was scanned at 532nm using an Affymetrix GeneCChip Scanner 3000
|
| Data processing |
Chip-quality was controled with Affymetrix expression console. CEL files were normalized and summarized using RMA.
|
| |
|
| Submission date |
Dec 15, 2011 |
| Last update date |
Feb 01, 2013 |
| Contact name |
Benita Wolf |
| Organization name |
University Hospital Tübingen
|
| Department |
Medical Clinic 1
|
| Lab |
AG Malek
|
| Street address |
Quenstedtstrasse 12
|
| City |
Tübingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE34465 |
Expression data from periphereal blood lymphocytes of metastatic renal cell carcinoma patients pre and post therapy as well as 9 healthy controls |
|
