|
| Status |
Public on Nov 22, 2024 |
| Title |
Healthy donor_003 |
| Sample type |
RNA |
| |
|
| Source name |
Human bone marrow biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Healthy donor
|
| Treatment protocol |
Bone marrow biopsies were used directly for RNA extraction.
|
| Growth protocol |
Bone marrow biopsies were harvested from the patient's iliac crest.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Bone marrow biopsy
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Sep 06, 2024 |
| Last update date |
Nov 22, 2024 |
| Contact name |
Yan Cheng |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Arkansas for Medical Sciences
|
| Department |
Internal Medicine
|
| Lab |
Fenghuang Zhan Lab
|
| Street address |
4301 W. Markham St.
|
| City |
Little Rock |
| State/province |
Arkansas |
| ZIP/Postal code |
72205 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE276561 |
Identifying bone marrow microenvironmental alterations in multiple myeloma and its precursor conditions using whole bone marrow biopsies |
|
