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Sample GSM8501344 Query DataSets for GSM8501344
Status Public on Nov 22, 2024
Title Smoldering multiple myeloma_032
Sample type RNA
 
Source name Human bone marrow biopsy
Organism Homo sapiens
Characteristics tissue: Smoldering multiple myeloma
Treatment protocol Bone marrow biopsies were used directly for RNA extraction.
Growth protocol Bone marrow biopsies were harvested from the patient's iliac crest.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Bone marrow biopsy
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Sep 06, 2024
Last update date Nov 22, 2024
Contact name Yan Cheng
E-mail(s) [email protected]
Organization name University of Arkansas for Medical Sciences
Department Internal Medicine
Lab Fenghuang Zhan Lab
Street address 4301 W. Markham St.
City Little Rock
State/province Arkansas
ZIP/Postal code 72205
Country USA
 
Platform ID GPL570
Series (1)
GSE276561 Identifying bone marrow microenvironmental alterations in multiple myeloma and its precursor conditions using whole bone marrow biopsies

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 685
1053_at 594
117_at 579
121_at 1856
1255_g_at 33
1294_at 1328
1316_at 318
1320_at 39
1405_i_at 2457
1431_at 102
1438_at 71
1487_at 706
1494_f_at 331
1552256_a_at 530
1552257_a_at 2481
1552258_at 624
1552261_at 35
1552263_at 1017
1552264_a_at 1218
1552266_at 70

Total number of rows: 54613

Table truncated, full table size 776 Kbytes.




Supplementary file Size Download File type/resource
GSM8501344_SMM032.CEL.gz 5.6 Mb (ftp)(http) CEL

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