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| Status |
Public on Jan 05, 2007 |
| Title |
FNR- mutant vs. WT (Dye swap-2) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
WT
|
| Organism |
Salmonella enterica subsp. enterica serovar Typhimurium |
| Characteristics |
strain: ATCC14028s, OD600=0.3, anaerobic, LB medium, 20 mM Xylose
|
| Extracted molecule |
total RNA |
| Label |
cy3
|
| |
|
| Channel 2 |
| Source name |
FNR null mutant
|
| Organism |
Salmonella enterica subsp. enterica serovar Typhimurium |
| Characteristics |
strain: NC983, OD600=0.3, anaerobic, LB medium, 20 mM Xylose
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| |
|
| |
| Description |
The labelling of the total RNA was performed using indirect labelling with AA-dUTP and subsequent coupling.
|
| Data processing |
Scanarray 4.0 has been used. Lowess normalization.
|
| |
|
| Submission date |
Nov 23, 2005 |
| Last update date |
Nov 14, 2006 |
| Contact name |
Ryan Cristiano Fink |
| E-mail(s) |
[email protected]
|
| Organization name |
St. Cloud State University
|
| Department |
Biology
|
| Lab |
Ryan C. Fink
|
| Street address |
720 4th Ave S
|
| City |
St. Cloud |
| State/province |
MN |
| ZIP/Postal code |
56301 |
| Country |
USA |
| |
|
| Platform ID |
GPL1835 |
| Series (1) |
| GSE3657 |
FNR- vs WT in anoxic conditions |
|
| Data table header descriptions |
| ID_REF |
identifier reference column |
| RATIO |
(Cy5 Normalized-Backgroung)/(Cy3 Normalized-Background) |
| VALUE |
-[INV_VALUE] |
| CH1_RAW |
raw signal output for channel 1 |
| CH1_BKD |
background signal output for channel 1 |
| CH1_SD |
standard deviation calculated for the mean signal for channel 1 |
| CH1_BKD_SD |
standard deviation for the mean background signal for channel 1 |
| CH2_RAW |
raw signal output for channel 2 |
| CH2_BKD |
background signal output for channel 2 |
| CH2_SD |
standard deviation calculated for the mean signal for channel 2 |
| CH2_BKD_SD |
standard deviation for the mean background signal for channel 2 |
| INV_VALUE |
log ratio (log2 of RATIO) |
| Data table |
| ID_REF |
RATIO |
VALUE |
CH1_RAW |
CH1_BKD |
CH1_SD |
CH1_BKD_SD |
CH2_RAW |
CH2_BKD |
CH2_SD |
CH2_BKD_SD |
INV_VALUE |
| 1 |
0.223057852 |
2.16451 |
52494 |
873 |
23029.31 |
638.49 |
12660 |
688 |
8330.23 |
324.55 |
-2.164510161 |
| 2 |
1.192575059 |
-0.25408 |
4025 |
837 |
1665.96 |
604.07 |
4628 |
675 |
1576.14 |
344.23 |
0.25408007 |
| 3 |
1.049462123 |
-0.0696501 |
2027 |
952 |
915.87 |
623.42 |
1961 |
788 |
1821.96 |
385.92 |
0.069650098 |
| 4 |
0.915490984 |
0.127382 |
4074 |
916 |
1641.53 |
554.54 |
3796 |
790 |
1381.6 |
379.98 |
-0.127382417 |
| 5 |
0.742898432 |
0.428763 |
2660 |
898 |
1587.02 |
598.74 |
2107 |
746 |
967.58 |
367.63 |
-0.428763113 |
| 6 |
0.763750522 |
0.388827 |
2383 |
858 |
1202.07 |
582.88 |
2005 |
794 |
862.87 |
375.86 |
-0.388826633 |
| 7 |
0.867628117 |
0.204851 |
5966 |
1022 |
2242.4 |
731.23 |
5250 |
790 |
1924.17 |
373.2 |
-0.204851289 |
| 8 |
0.92987413 |
0.104893 |
3862 |
1089 |
1526.51 |
674.15 |
3449 |
768 |
1390.54 |
350.57 |
-0.104892653 |
| 9 |
0.69424497 |
0.526483 |
3829 |
989 |
2737.62 |
666.67 |
2782 |
732 |
1240.56 |
365.76 |
-0.526483275 |
| 10 |
0.985797536 |
0.0206367 |
4836 |
871 |
1975.74 |
583.48 |
4828 |
764 |
1978.91 |
372.14 |
-0.020636719 |
| 11 |
1.094511594 |
-0.130287 |
12900 |
806 |
3561.69 |
557.42 |
14521 |
758 |
5719.65 |
342.91 |
0.130287237 |
| 12 |
1.806000269 |
-0.852798 |
6587 |
885 |
2475.27 |
583.77 |
11534 |
827 |
4063.04 |
419.13 |
0.852798107 |
| 13 |
0.836463524 |
0.257625 |
55590 |
1008 |
20994.21 |
674.13 |
48353 |
883 |
24993.8 |
440.13 |
-0.257625465 |
| 14 |
0.459312103 |
1.12245 |
6696 |
902 |
2414.17 |
600.2 |
3569 |
802 |
1332.05 |
370.08 |
-1.122453296 |
| 15 |
1.185666318 |
-0.245698 |
1204 |
783 |
756.07 |
546.42 |
1262 |
743 |
496.4 |
378.4 |
0.24569805 |
| 16 |
0.501480301 |
0.995735 |
49623 |
830 |
14618.18 |
616.3 |
26171 |
730 |
11502.55 |
349.58 |
-0.995735065 |
| 17 |
1.11657552 |
-0.159081 |
7625 |
753 |
2171.38 |
621.72 |
8758 |
780 |
2514.31 |
411.01 |
0.159080831 |
| 18 |
0.888599203 |
0.170395 |
2983 |
854 |
1570.73 |
643.38 |
2616 |
649 |
1159.06 |
327.02 |
-0.170395248 |
| 19 |
1.068448895 |
-0.0955179 |
1891 |
818 |
1088.59 |
563.65 |
1934 |
742 |
1389.38 |
339.7 |
0.095517904 |
| 20 |
0.809748806 |
0.304454 |
1072 |
781 |
611.75 |
602.36 |
942 |
697 |
457.15 |
333.26 |
-0.304453659 |
Total number of rows: 5776
Table truncated, full table size 487 Kbytes.
| Supplementary data files not provided |
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