|
| Status |
Public on Jan 10, 2012 |
| Title |
DP (CD4+/8+) cells from Normal Thymus Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
DP (CD4+/8+) cells from Normal Thymus
|
| Organism |
Mus musculus |
| Characteristics |
genotype: WT
sample type: DP (CD4+/8+) cells from Notch1-IC over-expressing peripheral tumor (thymoma)
genetic background: WT C57/BLACK6
cell type: DP (CD4+/8+) cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy (QIAGEN) according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
100 ng total RNA converted to cRNA using Affymetrix GeneChip® 3' IVT cDNA Synthesis and in vitro transcription kit resulting into biotin-labeled cRNA, fragmented prior to hybridization (Affymetrix Expression Analysis Technical Manual, as described).
|
| |
|
| Hybridization protocol |
10 mcg of cDNA was hybridized for 16 hr to the Affymetrix array as described in the Expression Analysis Technical Manual. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
|
| Scan protocol |
Affymetrix GeneArray scanner 7G
|
| Description |
Gene expression data from DP (CD4+/8+) cells from Normal Thymus Replicate 2
|
| Data processing |
Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using Agilent GeneSpring GX10 program.
|
| |
|
| Submission date |
Dec 19, 2011 |
| Last update date |
Jan 10, 2012 |
| Contact name |
Panagiotis Ntziachristos |
| E-mail(s) |
[email protected]
|
| Organization name |
NYU MEDICAL SCHOOL
|
| Department |
PATHOLOGY
|
| Street address |
522 FIRST AVENUE
|
| City |
NEW YORK |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE34554 |
Notch1-driven transcriptional changes in a mouse model of T-ALL |
|
