|
| Status |
Public on Jul 13, 2012 |
| Title |
RCK8 LMO2 transfected, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
RCK8 DLBCL_LMO2 transfected
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: RCK8 DLBCL
cell type: diffuse large B cell lymphoma
transfected with: pIREShrGFP II-LMO2 plasmid
|
| Treatment protocol |
pIREShrGFP II LMO2 plasmids were constructed using standard molecular biology methods and used for generation of the Rck8 LMO2 stable transfected cell line
|
| Growth protocol |
Cell lines were grown in RPMI 1640 medium (Mediatech, Inc. Herdon, VA), supplemented with 10% fetal bovine serum, 2 mM glutamine (GIBCO BRL, Grand Island, NY), and penicillin/streptomycin (Mediatech, Inc. Herdon, VA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was isolated from transfected cells using the Trizol reagent (Invitrogen) according to the manufacturer’s instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the standard Affymetrix protocol.
|
| Scan protocol |
The microarrays were scannedusing a GenePix 4000A scanner (Molecular Devices) and the resulting images were analyzed with the software package GenePix Pro.
|
| Description |
lmo2_3
|
| Data processing |
Data were analyzed using the rma function of Bioconductor with custom CDF file mapping to Entrez gene from Brainarray (version 14)
|
| |
|
| Submission date |
Dec 20, 2011 |
| Last update date |
Jul 13, 2012 |
| Contact name |
Andrew Gentles |
| Organization name |
Stanford University
|
| Street address |
1201 Welch Road
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL15034 |
| Series (1) |
| GSE34576 |
Identification of LMO2 transcriptome and interactome in diffuse large B-cell lymphoma |
|
