|
| Status |
Public on Dec 30, 2011 |
| Title |
SCC_WT2 CD34low |
| Sample type |
RNA |
| |
|
| Source name |
SCC CD34low WT
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: TbRII(fl/fl)
cell type: cutaneous squamous cell carcinoma
|
| Treatment protocol |
Total RNAs were isolated from cells sorted directly into TrizolLS (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from200 ng total RNA.
|
| |
|
| Hybridization protocol |
Mouse 430A 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Addymetrix 3000 7G Scanner.
|
| Data processing |
The data were analyzed using Gene Pattern (Broad Institute) and pre-processed using ExpressionFileCreator using the RMA statistical expression algorythm with background subtraction.
|
| |
|
| Submission date |
Dec 30, 2011 |
| Last update date |
Dec 30, 2011 |
| Contact name |
Markus Schober |
| E-mail(s) |
[email protected]
|
| Organization name |
Rockefeller University
|
| Street address |
1230 York Ave
|
| City |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE29328 |
Expression data from squamous cell carcinoma stem cells, epidermal progenitor cells and hair follicle bulge stem cells |
|
