|
| Status |
Public on Jun 01, 2012 |
| Title |
RPE1 cell, control oligo transfect, 72h |
| Sample type |
RNA |
| |
|
| Source name |
RPE1 cell transfeted with control oligo for 72h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: hTERT-RPE1
genotype/variation: control
|
| Treatment protocol |
hTERT-RPE1 cells were transfected with control (Ctrl) or miR-129-3p (M129) oligos for 72 h using Lipofectimine RNAiMAX (Invitrogen)
|
| Growth protocol |
hTERT-RPE1 cell lines were cultured with DMEM/F-12 containing 10% FBS and 0.01mg/ml hygromycin B at 37°C, 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy Mini Kit (Qiagen), according to the manufacturer’s instruction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (Cat. AM1792, Ambion).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Genome U133 Plus 2.0 with constant rotation at the speed of 60 rpm. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
RPE1-Ctrl.CEL
Gene expression data from hTERT-RPE1 cell
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings, and RMA normalization was done using Affy Suit in Bioconductor Package.
|
| |
|
| Submission date |
Jan 04, 2012 |
| Last update date |
Jun 01, 2012 |
| Contact name |
Jingli Cao |
| E-mail(s) |
[email protected]
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Cell & Developmental Biology
|
| Lab |
Jingli Cao
|
| Street address |
413 E 69th ST, BRB-518
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE34846 |
miR-129-3p control cilia assembly by regulating CP110 and actin dynamics [miR-129-3p overexpression] |
|
