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Status
Public on Dec 01, 2005
Title
untreated 3 h replicate 2
Sample type
RNA
Source name
suspension cell culture, untreated, 3 h
Organism
Arabidopsis thaliana Characteristics
Arabidopsis suspension cell culture, cv Lansberg erecta, 4 days post sub-culturing, sample collected 3 h post-treatment with treatment starting 2 h into the light cycle of a 16 h light 8 h dark light cycle
Biomaterial provider
Plant Molecular Biology Group: Rachel Clifton, Ryan Lister, Pia Sappl
Extracted molecule
total RNA
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type Arabidopsis suspension cell culture, pale green in colour
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 01, 2005
Last update date
Dec 01, 2005
Contact name
Rachel Clifton
E-mail(s)
[email protected] Organization name
Plant Energy Biology, ARC Centre of Excellence
Department
Biochemistry, UWA
Lab
Plant Molecular Biology Group
Street address
4th Floor CMS Building M310, University of Western Australia
City
Crawley
State/province
Western Australia
ZIP/Postal code
6009
Country
Australia
Platform ID
GPL198 Series (1)
GSE3709 Cell culture treatment study
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
222.1
P
0.000225
AFFX-BioB-M_at
301
P
0.00007
AFFX-BioB-3_at
321.7
P
0.00011
AFFX-BioC-5_at
566.8
P
0.00006
AFFX-BioC-3_at
407.7
P
0.000052
AFFX-BioDn-5_at
672.6
P
0.000044
AFFX-BioDn-3_at
2727.5
P
0.000044
AFFX-CreX-5_at
5112.6
P
0.000044
AFFX-CreX-3_at
9324.5
P
0.000044
AFFX-DapX-5_at
328.6
P
0.000081
AFFX-DapX-M_at
662.1
P
0.00006
AFFX-DapX-3_at
998.8
P
0.000044
AFFX-LysX-5_at
54.9
P
0.000317
AFFX-LysX-M_at
73.8
P
0.002867
AFFX-LysX-3_at
89.9
P
0.000081
AFFX-PheX-5_at
71
P
0.000169
AFFX-PheX-M_at
74.3
P
0.00141
AFFX-PheX-3_at
157.2
P
0.000147
AFFX-ThrX-5_at
83
P
0.000972
AFFX-ThrX-M_at
153.2
P
0.000044
22810 586 Kbytes .Supplementary data files not provided
