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Status
Public on Dec 01, 2005
Title
untreated 3 h replicate 4
Sample type
RNA
Source name
suspension cell culture, untreated, 3 h
Organism
Arabidopsis thaliana
Characteristics
Arabidopsis suspension cell culture, cv Lansberg erecta, 4 days post sub-culturing, sample collected 3 h post-treatment with treatment starting 2 h into the light cycle of a 16 h light 8 h dark light cycle
Biomaterial provider
Plant Molecular Biology Group: Rachel Clifton, Ryan Lister, Pia Sappl
Extracted molecule
total RNA
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type Arabidopsis suspension cell culture, pale green in colour
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 01, 2005
Last update date
Dec 01, 2005
Contact name
Rachel Clifton
E-mail(s)
[email protected]
Organization name
Plant Energy Biology, ARC Centre of Excellence
Department
Biochemistry, UWA
Lab
Plant Molecular Biology Group
Street address
4th Floor CMS Building M310, University of Western Australia
City
Crawley
State/province
Western Australia
ZIP/Postal code
6009
Country
Australia
Platform ID
GPL198
Series (1)
GSE3709
Cell culture treatment study
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
301.8
P
0.000972
AFFX-BioB-M_at
285.8
P
0.000127
AFFX-BioB-3_at
324.8
P
0.000052
AFFX-BioC-5_at
552.3
P
0.000127
AFFX-BioC-3_at
422.7
P
0.00006
AFFX-BioDn-5_at
666.5
P
0.00006
AFFX-BioDn-3_at
2877.3
P
0.000044
AFFX-CreX-5_at
5422.8
P
0.000044
AFFX-CreX-3_at
8932.4
P
0.000052
AFFX-DapX-5_at
385.9
P
0.000095
AFFX-DapX-M_at
741.7
P
0.000052
AFFX-DapX-3_at
1117.7
P
0.000044
AFFX-LysX-5_at
66.4
P
0.002275
AFFX-LysX-M_at
76
P
0.004998
AFFX-LysX-3_at
132.3
P
0.000052
AFFX-PheX-5_at
129.6
P
0.00034
AFFX-PheX-M_at
81.3
P
0.011384
AFFX-PheX-3_at
148.2
P
0.000446
AFFX-ThrX-5_at
96
P
0.000297
AFFX-ThrX-M_at
172.5
P
0.000147
Total number of rows: 22810 Table truncated, full table size 586 Kbytes .Supplementary data files not provided