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Status
Public on Dec 01, 2005
Title
treated with N2 to replace air, 3 h, replicate 1
Sample type
RNA
Source name
suspension cell culture, N2 air replacement, 3 h
Organism
Arabidopsis thaliana Characteristics
Arabidopsis suspension cell culture, cv Lansberg erecta, 4 days post sub-culturing, sample collected 3 h post-treatment with treatment starting 2 h into the light cycle of a 16 h light 8 h dark light cycle
Biomaterial provider
Plant Molecular Biology Group: Rachel Clifton, Ryan Lister, Pia Sappl
Extracted molecule
total RNA
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type Arabidopsis suspension cell culture, pale green in colour
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 01, 2005
Last update date
Dec 01, 2005
Contact name
Rachel Clifton
E-mail(s)
[email protected] Organization name
Plant Energy Biology, ARC Centre of Excellence
Department
Biochemistry, UWA
Lab
Plant Molecular Biology Group
Street address
4th Floor CMS Building M310, University of Western Australia
City
Crawley
State/province
Western Australia
ZIP/Postal code
6009
Country
Australia
Platform ID
GPL198 Series (1)
GSE3709 Cell culture treatment study
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
269.7
P
0.000258
AFFX-BioB-M_at
255
P
0.000081
AFFX-BioB-3_at
283.4
P
0.00006
AFFX-BioC-5_at
560.5
P
0.000044
AFFX-BioC-3_at
392.2
P
0.000044
AFFX-BioDn-5_at
562.7
P
0.00007
AFFX-BioDn-3_at
2808.2
P
0.000044
AFFX-CreX-5_at
5626.7
P
0.000044
AFFX-CreX-3_at
9489
P
0.000044
AFFX-DapX-5_at
316.7
P
0.000052
AFFX-DapX-M_at
680.4
P
0.000052
AFFX-DapX-3_at
986
P
0.00007
AFFX-LysX-5_at
49.9
P
0.000169
AFFX-LysX-M_at
85.8
P
0.002867
AFFX-LysX-3_at
131.1
P
0.001102
AFFX-PheX-5_at
89.3
P
0.000147
AFFX-PheX-M_at
85.4
P
0.001593
AFFX-PheX-3_at
144.5
P
0.000195
AFFX-ThrX-5_at
92.6
P
0.000446
AFFX-ThrX-M_at
142.4
P
0.00007
22810 585 Kbytes .Supplementary data files not provided
