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Status
Public on Dec 01, 2005
Title
untreated 12 h replicate 1
Sample type
RNA
Source name
suspension cell culture, untreated, 12 h
Organism
Arabidopsis thaliana
Characteristics
Arabidopsis suspension cell culture, cv Lansberg erecta, 4 days post sub-culturing, sample collected 3 h post-treatment with treatment starting 2 h into the light cycle of a 16 h light 8 h dark light cycle
Biomaterial provider
Plant Molecular Biology Group: Rachel Clifton, Ryan Lister, Karen Parker
Extracted molecule
total RNA
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type Arabidopsis suspension cell culture, pale green in colour
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 01, 2005
Last update date
Dec 01, 2005
Contact name
Rachel Clifton
E-mail(s)
[email protected]
Organization name
Plant Energy Biology, ARC Centre of Excellence
Department
Biochemistry, UWA
Lab
Plant Molecular Biology Group
Street address
4th Floor CMS Building M310, University of Western Australia
City
Crawley
State/province
Western Australia
ZIP/Postal code
6009
Country
Australia
Platform ID
GPL198
Series (1)
GSE3709
Cell culture treatment study
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
186.5
P
0.000258
AFFX-BioB-M_at
263.7
P
0.000044
AFFX-BioB-3_at
185
P
0.000044
AFFX-BioC-5_at
453.9
P
0.000095
AFFX-BioC-3_at
246.8
P
0.00006
AFFX-BioDn-5_at
510.6
P
0.000044
AFFX-BioDn-3_at
2029.1
P
0.000044
AFFX-CreX-5_at
4304.8
P
0.000044
AFFX-CreX-3_at
6484.9
P
0.000044
AFFX-DapX-5_at
2.3
A
0.559354
AFFX-DapX-M_at
1.5
A
0.966323
AFFX-DapX-3_at
2.3
A
0.932322
AFFX-LysX-5_at
1.1
A
0.949771
AFFX-LysX-M_at
4.5
A
0.834139
AFFX-LysX-3_at
11.6
A
0.147939
AFFX-PheX-5_at
1.5
A
0.979978
AFFX-PheX-M_at
1.3
A
0.737173
AFFX-PheX-3_at
4.7
A
0.860518
AFFX-ThrX-5_at
8.1
A
0.559354
AFFX-ThrX-M_at
2.2
A
0.772364
Total number of rows: 22810 Table truncated, full table size 587 Kbytes .Supplementary data files not provided