Samples were dissected on ice and placed into RLT (Qiagen) at -80
Growth protocol
Flies were raised at 25C
Extracted molecule
polyA RNA
Extraction protocol
RNAeasy (Qiagen)
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
