Primary hepatic stellate cells were isolated from rodent liver and cultureed in DMEM with 10% fetal bovine serum and antibiotics for 7 days.
Growth protocol
Primary hepatic stellate cells were isolated C57BL6 mice, assessed for purity and viability and cultured for 7days.
Extracted molecule
total RNA
Extraction protocol
Agilent 2100 Bioanalyzer G2939A, Agilent Technologies (Santa Clara, CA) Nanodrop 8 spectrophotometer, Thermo Scientific/NanoDrop (Wilmington, DE) Combination of Trizol and Rneasy columns extraction of total RNA was performed according to the manufacturer's instructions.
Label
biotin
Label protocol
MessageAmp Premier RNA Amplification Kit (Part Number 4385821, AM1792, 4383452), Applied Biosystems/Ambion (Austin, TX)
