|
| Status |
Public on Nov 01, 2012 |
| Title |
HUH-nonsense-#2 |
| Sample type |
RNA |
| |
|
| Source name |
transfection with nonsense siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Hep3B
treatment: transfection with nonsense siRNA
|
| Treatment protocol |
Transfection of siRNAs was performed according to the Oligofectamine protocol provided by the company (Invitrogen).
|
| Growth protocol |
Hep3B cells were grwon under full media conditions for 24 hours. After transfection with siRNAs cells were cultivated for further 24 hours. RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Nucleospin RNAII II (Macherey-Nagel) was used for total RNA extraction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Hep3B cells transfected with nonsense siRNA
|
| Data processing |
The data were imported and analyzed with a commercial software called JMP Genomics, version 4, from SAS. The Raw fluorescence intensity values were normalized applying quantile normalization.
|
| |
|
| Submission date |
Jan 11, 2012 |
| Last update date |
Nov 01, 2012 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE35004 |
YAP Inhibition in HCC cells (Hep3B) |
|
