|
| Status |
Public on Nov 11, 2024 |
| Title |
ADAR1 and NOCT knockdown 3 |
| Sample type |
RNA |
| |
|
| Source name |
Pulmonary artery endothelial cell isolated from lungs
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Lung endothelial cells
genotype: wildtype
|
| Treatment protocol |
ADAR1 siRNAs, NOCT siRNAs, or non-specific siRNAs were tranfected into hPAECs for 48 hours
|
| Growth protocol |
primary human pulmonary arterial endothelial cells (hPAECs) were inculated at 37C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QiAzol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Affymetrix microarray
|
| |
|
| Hybridization protocol |
Affymetrix microarray
|
| Scan protocol |
Affymetrix microarray
|
| Data processing |
RMA
|
| |
|
| Submission date |
Nov 04, 2024 |
| Last update date |
Nov 11, 2024 |
| Contact name |
Shu-Ting Cho |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Pittsburgh
|
| Department |
Computational and Systems Biology
|
| Street address |
450 Technology Drive
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15219 |
| Country |
USA |
| |
|
| Platform ID |
GPL24539 |
| Series (1) |
| GSE281068 |
ADAR1 Deficiency Relies upon NOCT to Activate Endothelial Innate Immunity and Apoptosis. |
|
