|
| Status |
Public on Jan 20, 2012 |
| Title |
L1236 B_1 Un_1 (gene) |
| Sample type |
RNA |
| |
|
| Source name |
Human Hodgkin Lymphoma Cell Line
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: infected uninduced shREL-B
cell line: L1236
|
| Treatment protocol |
HL cell lines were spin-infected using a retrovirus that delivers a specific shRNA to knock down REL-B expression twice in consecutive days. Cell are split into two populations. One is infected and uninduced and the second one is treated with doxyciline to induce the expression of REL-B shRNA.
|
| Growth protocol |
Hodgkin Lymphoma cells are culture in RPMI medium supplemented with 20% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL
|
| Label |
biotin
|
| Label protocol |
Sense strand cDNA was fragmented and labeled using Affymetrix WT terminal labeling kit.
|
| |
|
| Hybridization protocol |
Samples wre hybridize to Affymetrix human Gene ST 1.0 GeneChIP in Affymetrix hybridization oven at 45C, 60 rpm for 16 hours.
|
| Scan protocol |
Slides were scanned on Affymetrix geneChip scanner 3000.
|
| Data processing |
Data were collected using Affymetrix AGCC software. Statistical and clustering analysis was performed with Partek genomics Suite software using RMA normalization algorithm.
|
| |
|
| Submission date |
Jan 19, 2012 |
| Last update date |
Jan 20, 2012 |
| Contact name |
Brian Andrew Lewis |
| E-mail(s) |
[email protected]
|
| Phone |
301-435-8323
|
| Fax |
301-496-9956
|
| Organization name |
National Cancer Institute-NIH
|
| Department |
metabolism Branch
|
| Lab |
Lewis Lab
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE35224 |
Gene expression profiling of Hodgkin Lymphoma cell line following REL-B depletion |
|
