|
| Status |
Public on Nov 28, 2024 |
| Title |
F_41F8_072921_blood_Control (long-term) |
| Sample type |
genomic |
| |
|
| Source name |
blood
|
| Organism |
Mus musculus |
| Characteristics |
tissue: blood
strain: C57BL/6J
treatment: Control (long-term)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using Dneasy blood and tissue kit (QIAGEN #69506). DNA samples were normalized to between 12.5-25 ng/μL in 30 μL. DNA was bisulfite converted using the Zymo EZ DNA methylation kit (D5004).
|
| Label |
Cy3 and Cy5
|
| Label protocol |
A Tecan liquid handler was used to automate the process from Hybridization to X-Stain
|
| |
|
| Hybridization protocol |
A Tecan liquid handler was used to automate the process from Hybridization to X-Stain
|
| Scan protocol |
Samples were scanned on the Illumina iScan System
|
| Description |
F_41F8_072921
|
| Data processing |
The SeSaMe normalization method was used to estimate methylation levels (B-values) for each CpG site.
processed value definition: B-values provide the average methylation levels for each CpG site. Detection P-values represent the probability of a detected signal being background fluorescence
|
| |
|
| Submission date |
Nov 21, 2024 |
| Last update date |
Nov 28, 2024 |
| Contact name |
Sinisa Hrvatin |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
MIT
|
| Department |
Whitehead Institute
|
| Lab |
Hrvatin Lab
|
| Street address |
455 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL32543 |
| Series (1) |
| GSE282499 |
A torpor-like state in mice slows blood epigenetic aging and prolongs healthspan [9155] |
|
