|
| Status |
Public on Nov 27, 2024 |
| Title |
KYSE30 cells, shKAT8 |
| Sample type |
SRA |
| |
|
| Source name |
KYSE30
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: KYSE30
cell type: Human esophageal carcinoma
genotype: KAT8 knockdown
treatment: Virus infection
|
| Growth protocol |
KYSE30 cells were maintained in RPMI1640 supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidifined atmosphere with 5% CO2 at 37 ℃
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol. 1ug of total RNA was used for the construction of sequencing libraries
RNA libraries for RNA-seq were prepared using Hieff NGS® Ultima Dual-mode mRNA Library Prep Kit following manufacturer's protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq X Plus |
| |
|
| Description |
Library name: Sample 2
|
| Data processing |
Raw reads were controlled by FASTP to filter out low-quality data,then obtain clean reads. The alignment tool Bowtie2 was used to align the clean reads to the human ribosome database, and the retained unmapped reads were used for subsequent transcriptome data. HISAT2 software was used for reference genome-based alignment analysis
Assembly: Ensembl_release 111
Supplementary files format and content: tab-delimited text file includes TPM values for each sample
|
| |
|
| Submission date |
Nov 22, 2024 |
| Last update date |
Nov 27, 2024 |
| Contact name |
Dandan Zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
China-US (Henan) hormel cancer insititute
|
| Street address |
Dongming road
|
| City |
Zhengzhou |
| ZIP/Postal code |
450000 |
| Country |
China |
| |
|
| Platform ID |
GPL34284 |
| Series (1) |
| GSE282588 |
CHI-KAT8i5 suppresses ESCC tumor growth through inhibiting KAT8 mediate c-Myc stability |
|
| Relations |
| BioSample |
SAMN44960092 |
| SRA |
SRX26810318 |
