|
| Status |
Public on Nov 27, 2024 |
| Title |
CG1603PBac mutant larvae, biol rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
larva
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: larva
genotype: CG1603PBac mutant
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted by Trizol (Thermo Fisher Scientific) following standard protocol. Contaminating DNA was removed by DNA-free™ DNA Removal Kit (Thermo Fisher Scientific). 1µg total RNA used for library construction.
Poly (A) capture libraries were generated at the DNA Sequencing and Genomics Core, NHLBI, NIH following standard protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Library name: CG1603PBac2
|
| Data processing |
Bioinformatics Pipeline of mRNA Analysis, NCI, NIH.
Quality assessment of FASTQ files using FastQC
Aligned against Drosophila Melanogaster reference genome using a two-pass method with STAR
Gene level read counts were produced by HTseq
Assembly: dm6
Supplementary files format and content: Output text files generated by HTseq containing raw counts for each sample
|
| |
|
| Submission date |
Nov 22, 2024 |
| Last update date |
Nov 27, 2024 |
| Contact name |
Fan Zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
NHLBI
|
| Street address |
10 Center Dr.
|
| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL25244 |
| Series (1) |
| GSE282638 |
CG1603 regulation of nuclear-encoded mitochondrial gene expression |
|
| Relations |
| BioSample |
SAMN44979917 |
| SRA |
SRX26816570 |
