NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM86525 Query DataSets for GSM86525
Status Public on Dec 09, 2005
Title 3D3, chip MG_U74Av2
Sample type RNA
 
Source name Mesenchymal cells, Limb bud
Organism Mus musculus
Characteristics Strain: CD-1
Defining markers: Sox9, type II collagen
Treatment protocol Assays to confirm viability: Cells were allowed to differentiate <i>in vitro</i> into chondrocytes
Extracted molecule total RNA
Extraction protocol RNA prep method: RNeasy
Cell purification method: Cells were isolated from the subridge region of the developing limb
Contaminating cell type: Fibroblasts
Method for estimating purity: Morphology of cultures 3 days post harvest
Label Biotin
 
Description This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. T. Michael Underhill ([email protected]; 1400 Western Road) for analysis.
Stembase Experiment ID: E30
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=30
SCGP Sample ID: S34
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=30#SAMPLE_32
Short description: Chondroblasts harvested at 3 days.
Estimated purity: 90-95%
Num cells for RNA prep: 6x10e6
Data processing Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
 
Submission date Dec 07, 2005
Last update date Jan 19, 2006
Organization Ottawa Hospital Research Institute
Phone (613) 737-8899 -73255
Department Cellular and Molecular Medicine
Lab Ottawa Bioinformatics Core Facility
Street address 501 Smyth Rd.
City Ottawa
State/province ON
ZIP/Postal code K1H 8L6
Country Canada
 
Platform ID GPL81
Series (1)
GSE3770 Temporal Analysis of Differentiating Limb Bud Mesenchymal Cell Gene Expression

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 118.376 A 0.824672
AFFX-MurIL10_at 130.628 A 0.60308
AFFX-MurIL4_at 79.7182 A 0.470241
AFFX-MurFAS_at 408.307 P 0.0284573
AFFX-BioB-5_at 1951.29 P 0.0020226
AFFX-BioB-M_at 4602.36 P 5.16732e-05
AFFX-BioB-3_at 2733.83 P 0.000169227
AFFX-BioC-5_at 6239.95 P 0.000126798
AFFX-BioC-3_at 6195 P 4.42873e-05
AFFX-BioDn-5_at 6227.67 P 7.00668e-05
AFFX-BioDn-3_at 31305.7 P 7.00668e-05
AFFX-CreX-5_at 72181.1 P 4.42873e-05
AFFX-CreX-3_at 93820 P 4.42873e-05
AFFX-BioB-5_st 255.641 A 0.205732
AFFX-BioB-M_st 124.577 A 0.631562
AFFX-BioB-3_st 91.2851 A 0.672921
AFFX-BioC-5_st 72.3493 A 0.860518
AFFX-BioC-3_st 70.7921 A 0.659339
AFFX-BioDn-5_st 542.624 A 0.156732
AFFX-BioDn-3_st 1264.56 P 0.026111

Total number of rows: 12488

Table truncated, full table size 364 Kbytes.




Supplementary file Size Download File type/resource
GSM86525.CEL.gz 2.7 Mb (ftp)(http) CEL
GSM86525.EXP.gz 518 b (ftp)(http) EXP

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap