Growth medium: Stem span Media supplements: Thrombopoetin, Flt-3 ligand, stem cell factor Culture conditions: 37 C, 5% CO2
Extracted molecule
total RNA
Extraction protocol
RNA prep method: TRIzol
Label
Biotin
Description
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Peter Zandstra ([email protected]; 170 College Street) for analysis. Stembase Experiment ID: E130 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=130 SCGP Sample ID: S171 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=130#SAMPLE_131 Short description: Cord blood derived, lineage negative, hematopoietic cells were cultured for 4 days and then subjected to a mock medium exchange. This included centrifugation of the cells and re-suspension in the same medium. The cells were then allowed to incubate for 24 hours prior to RNA extraction. RNA concentration: 50 ng/ul Num cells for RNA prep: 1.8x10e6 Sample volume: 3.0 ul
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
