|
| Status |
Public on Feb 01, 2012 |
| Title |
Key biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Key biological rep 2
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Whole fly
strain: w1118
genotype: key1
|
| Treatment protocol |
4-5 day old flies were placed in a fly vial with a cellulose plug at the bottom. 2mls of LB broth containing 10E8 V. cholerae per ml was added to the plug. Flies were allowed to ingest the LB mixture for 24 hours and then RNA was extracted
|
| Growth protocol |
flies were raised in standard fly media at 24 degrees C prior to use in experiments
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Flies were homogenized in Trizol reagent. One hundred flies were used per array. Total RNA was extracted once, treated with DNaseI, and extracted again. This was supplied to a core facility for hybridization to Affymetrix GeneCHIP Drosophila 2.0
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol
|
| Data processing |
Data processing was done using Gene Spring software. Statistical significance was determined using a one-way ANOVA with a threshold p value of 0.05
|
| |
|
| Submission date |
Jan 31, 2012 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Paula Watnick |
| E-mail(s) |
[email protected]
|
| Phone |
617-919-2918
|
| Organization name |
Children's Hospital, Boston
|
| Department |
Infectious Diseases
|
| Street address |
300 Longwood Avenue
|
| City |
Boston |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE35439 |
Differences in gene expression of Mustard and Kenny mutants relative to control w1118 flies 24 hours after access to food contaminated with Vibrio cholerae |
|
| Relations |
| Reanalyzed by |
GSE119084 |
