|
| Status |
Public on Feb 20, 2012 |
| Title |
eyedisc_control_biological replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila L3 control eyedisc
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: w1118
genotype: GS(2)88A8 UAS-Dl/+
developmental stage: larva 3
|
| Treatment protocol |
none
|
| Growth protocol |
Drosophila larvae were grown at 25ºC until wandering stage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
larvae were rinsed in PBS. Discs were dissected in the same buffer, transferred to RNA later and total RNA was isolated with the Trizol reagent and the RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
After DNAse digestion with RNAse-free Dnase set (Qiagen), cDNA was synthesized and purified with the One cycle cDNA synthesis kit (Affymetrix). The cRNA obtained was marked and purified with the IVT kit (Affymetrix), and 15 ug of cRNA were hybridized to Drosophila Genome 2.0 arrays (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
Gene Chip microarray scan protocol (Affymetrix Expression Analysis Technical Manual).
|
| Data processing |
The Affymetrix data were analyzed using CARMAweb tools and the raw data were processed with the gcRMA method. Differentially expressed genes were detected by SAM (Significance Analysis of Microarrays) with a 0.1378 FDR, and using the Benjamini and Hochberg correction for multiple hypothesis testing.
|
| |
|
| Submission date |
Feb 01, 2012 |
| Last update date |
Aug 28, 2018 |
| Contact name |
andres garelli |
| E-mail(s) |
[email protected]
|
| Organization name |
instituto de neurociencias de alicante
|
| Street address |
c/Ramón y Cajal s/n
|
| City |
San Juan de Alicante |
| ZIP/Postal code |
03550 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE35471 |
Expression data from L3 Drosophila antennal-eyediscs |
|
| Relations |
| Reanalyzed by |
GSE119084 |
