|
| Status |
Public on Mar 01, 2012 |
| Title |
Adipocytes_Hu_2 |
| Sample type |
RNA |
| |
|
| Source name |
subcutaneous preadipocytes differentiated 14d
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: subcutaneous preadipocytes
medium: adipogenic
group: adipo-ref
|
| Treatment protocol |
control and reference cultures maintained in specified media and split when confluent. Differentiated fibroblasts kept in induction media for 4 weeks without passaging.
|
| Growth protocol |
all cells cultured and expanded in T75 plastic culture flasks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cultures were trypsinized, RNA extracted with Macherey-Nagel Total RNA isolation kit (filter-column kit). Checked with NanoDrop and Agilent BioAnalyzer 2000.
|
| Label |
biotin
|
| Label protocol |
Labelled according to Affymetrix HuGene 1.0ST protocol 2009.
|
| |
|
| Hybridization protocol |
Hybridization according to Affymetrix HuGene 1.0ST protocol 2009
|
| Scan protocol |
According to protocol. Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
AC-pa-AM_2
|
| Data processing |
CEL-files imported into Agilent GeneSpring GX11 and 11.5. Quantile normalization, baseline to median of all samples using RMA16 (core). Data exported as tab-delimited txt showing transcript cluster id and normalized intensity values.
Affymetrix.ExonExprChip.HuGene-1_0-st-v1
|
| |
|
| Submission date |
Feb 03, 2012 |
| Last update date |
Mar 01, 2012 |
| Contact name |
Stefan Thor |
| E-mail(s) |
[email protected]
|
| Organization name |
The University of Queensland
|
| Department |
School of Biomedical Sciences
|
| Street address |
Skerman Bldg (65) room 506 Faculty of Medicine
|
| City |
Brisbane |
| State/province |
QLD |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE35545 |
Fibroblasts under adipogenic, chondrogenic or osteogenic differentiation medium and controls |
|
