|
| Status |
Public on Apr 05, 2012 |
| Title |
RpII2154/FM7 ovaries at 20oC- biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
RpII2154/FM7 (20oC)
|
| Organism |
Drosophila melanogaster |
| Characteristics |
gender: female
tissue: ovary
genotype: RpII2154/FM8
temperature: 20oC
|
| Treatment protocol |
Flies were either untreated (20oC) or heat stressed (25oC).
|
| Growth protocol |
Flies from different genotypes were reared either at 20oC or 25oC incubators.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN RNeasy Mini Kit
|
| Label |
biotin
|
| Label protocol |
One-cycle target labeling
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
3663-2-SR100514CBL040-2_01.CEL
|
| Data processing |
Bioconductor RMA
The yw expression data from both the base and high temperature states were used to determine a set of expressed transcripts using the maximum RMA value for each probe set across all individual yw samples. Probe sets were identified as being expressed when the maximum RMA value was greater than or equal to six.
|
| |
|
| Submission date |
Feb 03, 2012 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Alireza Baradaran-Heravi |
| E-mail(s) |
[email protected]
|
| Organization name |
UBC
|
| Department |
Medical Genetics
|
| Street address |
950 West 28th Avenue, Room 218
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 4H4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE35552 |
Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression (2) |
| GSE35554 |
Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression |
|
| Relations |
| Reanalyzed by |
GSE119084 |
