|
| Status |
Public on Mar 01, 2012 |
| Title |
Free-living mycelium of Laccaria bicolor S238N Nut 10d-2 |
| Sample type |
RNA |
| |
|
| Source name |
Free-living mycelium of Laccaria bicolor S238N Nut 10d
|
| Organism |
Laccaria bicolor |
| Characteristics |
sample type: Free-living mycelium of Laccaria bicolor S238N precultured for 15 days on MMN medium was transferred tonto agar medium supplemented with the same nutrients used to fertilize our mycorrhization experiments and grown for another 10 days
tissue: Free-living mycelium
growth condition: precultured for 15 days on MMN medium was transferred tonto agar medium supplemented with the same nutrients used to fertilize our mycorrhization experiments and grown for another 10 days
|
| Biomaterial provider |
Institut National de la Recherche Agronomique (INRA Nancy, France)
|
| Growth protocol |
For nutrient deprivation experiments, L. bicolor colonies (grown on cellophane membranes) were started on MMN media for 15 days after which they were transferred onto agar medium
supplemented with the same nutrients used to fertilize our myco-
rrhization experiments (2.5 mM KNO3, 0.8 mM KH2PO4, 1mM
MgSO4x7H2O, 2.3 mM Ca(NO3)2x4H2O, 23 µM H3BO3, 4.6 µM
MnCl2x4H2O, 0.4 ZnSO4x7H2O, 0.09 µM (NH4)2MoO4, 0.18 µM CuSO4x5H2O, 20 µM FeNaEDTA, pH 5.8). Samples were harvested after another 10d in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was carried out using the RNeasy Plant Mini Kit (Qiagen). Total RNA preparations
were amplified using the SMART PCR cDNA Synthesis Kit (Clontech) according to the
manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Labelling by NimbleGen Company
|
| |
|
| Hybridization protocol |
Hybridization by NimbleGen Company
|
| Scan protocol |
Scanning by NimbleGen Company
|
| Data processing |
Expression values reflect
across-array quantile normalization and gene calls generated using the Robust Multichip Average
(RMA). For information on RMA see Biostatistics 2003 Apr; 4(2): 249-264
|
| |
|
| Submission date |
Feb 06, 2012 |
| Last update date |
Mar 01, 2012 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
[email protected]
|
| Phone |
+33 (0)383 394072
|
| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
| |
|
| Platform ID |
GPL14641 |
| Series (3) |
| GSE35556 |
Gene expression of Laccaria bicolor S238N free-living mycelium grown on different media |
| GSE62225 |
Laccaria-Poplar: A Timecourse of Mycorrhizal Root Tip Development |
| GSE62226 |
Laccaria-Douglas fir: A Timecourse of Mycorrhizal Root Tip Development |
|
