|
| Status |
Public on Dec 12, 2006 |
| Title |
P.aeruginosa_+40µMPQS_20h_2 |
| Sample type |
RNA |
| |
|
| Source name |
P. aeruginosa total RNA after 20 h growth with 40 µM PQS
|
| Organism |
Pseudomonas aeruginosa PAO1 |
| Characteristics |
In order to evaluate the influence of 40µM PQS on the global Pseudomonas aeruginosa gene expression, we performed a comparative gene expression analysis of PQS-treated versus untreated P. aeruginosa PAO1 cultures after 5, 11 and 20 h of incubation.
|
| Treatment protocol |
P. aeruginosa PAO1 cultures with addition of 40 µM PQS were grown in BHI medium and total RNA was extracted after 20 h of growth
|
| Growth protocol |
P. aeruginosa PAO1 cultures with addition of 40 µM PQS were grown in 10 ml BHI medium in a 50 ml flask shaking culture (180 rpm, 37°C) and total RNA was extracted after 20 h of growth
|
| Extracted molecule |
total RNA |
| Label |
Biotin-16-ddUTP
|
| |
|
| Hybridization protocol |
Hybridization of the probes was carried out according to the manufacturer’s “Expression Analysis Protocol (p. 25: Modified Fluidic Protocol for P. aeruginosa cDNA Assay)”, see http://www.affymetrix.com:
|
| Description |
· Threshold of Signal Log ratio: -1/1; change is I (increased), MI (marginally increased), D (decreased), MD (marginally decreased); change p-value >0.999 or <0.001; only genes that are present (Detection=P) in at least one of the compared chipsets.
|
| Data processing |
MAS 5.0
|
| |
|
| Submission date |
Dec 12, 2005 |
| Last update date |
Dec 15, 2005 |
| Contact name |
Florian Bredenbruch |
| E-mail(s) |
[email protected]
|
| Organization name |
GBF-Braunschweig
|
| Department |
Cellbiology
|
| Lab |
CPI
|
| Street address |
Mascheroderweg 1
|
| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE3836 |
Transcriptome analysis of PAO1 cultures supplemented with PQS |
|
