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Sample GSM875423 Query DataSets for GSM875423
Status Public on Jul 05, 2012
Title gastric tumor Gastric0020
Sample type RNA
 
Source name gastric tumor Gastric0020
Organism Homo sapiens
Characteristics tissue: gastric adenocarcinoma
subtype: Invasive
Treatment protocol gastric tumor tissue.
Growth protocol gastric tumor tissue.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from primary tumors using RNeasy mini spin columns (Qiagen) according to the instructions of the manufacturer.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 to 5 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000 7G.
Description gene expression data from gastric tumor Gastric0020
Data processing The data were RMA normalized with R package affy.
 
Submission date Feb 14, 2012
Last update date Jul 05, 2012
Contact name Zhengdeng Lei
E-mail(s) [email protected]
Organization name Duke-NUS graduate medical school
Lab Lei
Street address 8 College Road
City Singapore
ZIP/Postal code 169857
Country Singapore
 
Platform ID GPL570
Series (1)
GSE35809 Gastric Cancer Subtyping (Australian Patient Cohort)

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
1007_s_at 9.26208256
1053_at 6.665576666
117_at 6.358635218
121_at 7.212310216
1255_g_at 3.38935945
1294_at 7.705960557
1316_at 5.241886145
1320_at 5.086582403
1405_i_at 8.08849
1431_at 5.56835337
1438_at 6.588147565
1487_at 7.231795469
1494_f_at 4.804748703
1552256_a_at 7.240973229
1552257_a_at 7.781857942
1552258_at 4.532817096
1552261_at 4.284596206
1552263_at 5.962055017
1552264_a_at 7.030759044
1552266_at 3.490530096

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM875423.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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