|
| Status |
Public on Dec 28, 2012 |
| Title |
miR-214_replicate3 |
| Sample type |
RNA |
| |
|
| Source name |
Myeloma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H929
transfection: miR-214
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from the cell lines using Trizol reagent (Invitrogen) according to the standard protocol. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent Tech, Palo Alto, CA, USA).
|
| Label |
Biotin
|
| Label protocol |
Labelling and hybridizations were performed according to protocols from Affymetrix. Briefly, 100-300 ng of total RNA were amplified and labeled using the WT Sense Target labelling and control reagents kit (Affymetrix Inc., Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized to Human Gene 1.0 ST Array
|
| Scan protocol |
Washing and scanning were performed using GeneChip System of Affymetrix (GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450 and GeneChip Scanner 7G).
|
| Description |
H929 cell line transfected with non-targeting control
|
| Data processing |
Expression value for each probe set was calculated using dChip program that uses Li and Wong algorithm.
|
| |
|
| Submission date |
Feb 17, 2012 |
| Last update date |
Dec 28, 2012 |
| Contact name |
Norma Carmen Gutierrez |
| E-mail(s) |
[email protected]
|
| Phone |
34923291375
|
| Organization name |
University Hospital
|
| Department |
Hematology
|
| Street address |
|
| City |
Salamanca |
| ZIP/Postal code |
37007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE35948 |
Restoration of miR-214 expression reduces cell growth of myeloma cells through a positive regulation of P53 and inhibition of DNA replication |
|
