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Sample GSM87729 Query DataSets for GSM87729
Status Public on Jan 17, 2006
Title BM +/- 1, chip HG-U133A
Sample type RNA
 
Source name Hematopoietic stem cells, Bone marrow
Organism Homo sapiens
Characteristics Defining markers: Lin-, CD34+, CD38-
Treatment protocol Assays to confirm viability: 7AAD staining
Extracted molecule total RNA
Extraction protocol RNA prep method: RNeasy
Cell purification method: 1. Ficoll separation to isolate mononuclear cells., 2. Lineage depleted cocktail to enrich progenitors.</p>3. CD34 and CD38 fluorochromes to enrich hematopoietic stem cells using FACS-Vantage.
Method for estimating purity: Purity was checked after sorting the cells on FACS-Vantage.
Label Biotin
 
Description This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Mickie Bhatia ([email protected]; Box 5015; 100 Perth Drive) for analysis.
Stembase Experiment ID: E43
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=43
SCGP Sample ID: S144
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=43#SAMPLE_106
Short description: Amplified RNA from bone marrow.
Estimated purity: > 98%
RNA concentration: 500 ng/ul
Num cells for RNA prep: 200,000
Sample volume: 30.0 ul
Data processing Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
 
Submission date Dec 14, 2005
Last update date Mar 01, 2006
Organization Ottawa Hospital Research Institute
Phone (613) 737-8899 -73255
Department Cellular and Molecular Medicine
Lab Ottawa Bioinformatics Core Facility
Street address 501 Smyth Rd.
City Ottawa
State/province ON
ZIP/Postal code K1H 8L6
Country Canada
 
Platform ID GPL96
Series (1)
GSE3823 Molecular Signatures Orchestrating fate of Human Hematopoietic Stem Cells Originating From Different Stages of Ontogeny

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 5756.01 P 0.000340305
AFFX-BioB-M_at 10548.5 P 4.42873e-05
AFFX-BioB-3_at 9216.75 P 0.000169227
AFFX-BioC-5_at 12803.7 P 7.00668e-05
AFFX-BioC-3_at 11627.5 P 4.42873e-05
AFFX-BioDn-5_at 15282.3 P 4.42873e-05
AFFX-BioDn-3_at 78145.3 P 5.16732e-05
AFFX-CreX-5_at 165123 P 4.42873e-05
AFFX-CreX-3_at 230910 P 4.42873e-05
AFFX-DapX-5_at 36.5465 A 0.631562
AFFX-DapX-M_at 58.0349 A 0.760937
AFFX-DapX-3_at 38.697 A 0.953518
AFFX-LysX-5_at 148.165 A 0.116113
AFFX-LysX-M_at 284.531 A 0.51489
AFFX-LysX-3_at 14.139 A 0.724854
AFFX-PheX-5_at 32.4185 A 0.910522
AFFX-PheX-M_at 60.9033 A 0.804734
AFFX-PheX-3_at 98.7266 A 0.783476
AFFX-ThrX-5_at 58.7211 A 0.672921
AFFX-ThrX-M_at 59.4395 A 0.737173

Total number of rows: 22283

Table truncated, full table size 660 Kbytes.




Supplementary file Size Download File type/resource
GSM87729.CEL.gz 3.2 Mb (ftp)(http) CEL
GSM87729.EXP.gz 500 b (ftp)(http) EXP

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