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Status
Public on Jan 17, 2006
Title
BM +/- 1, chip HG-U133A
Sample type
RNA
Source name
Hematopoietic stem cells, Bone marrow
Organism
Homo sapiens
Characteristics
Defining markers: Lin-, CD34+, CD38-
Treatment protocol
Assays to confirm viability: 7AAD staining
Extracted molecule
total RNA
Extraction protocol
RNA prep method: RNeasy Cell purification method: 1. Ficoll separation to isolate mononuclear cells., 2. Lineage depleted cocktail to enrich progenitors.</p>3. CD34 and CD38 fluorochromes to enrich hematopoietic stem cells using FACS-Vantage. Method for estimating purity: Purity was checked after sorting the cells on FACS-Vantage.
Label
Biotin
Description
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/ ). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/ ) by Dr. Mickie Bhatia ([email protected] ; Box 5015; 100 Perth Drive) for analysis. Stembase Experiment ID: E43 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=43 SCGP Sample ID: S144 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=43#SAMPLE_106 Short description: Amplified RNA from bone marrow. Estimated purity: > 98% RNA concentration: 500 ng/ul Num cells for RNA prep: 200,000 Sample volume: 30.0 ul
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
Submission date
Dec 14, 2005
Last update date
Mar 01, 2006
Organization
Ottawa Hospital Research Institute
Phone
(613) 737-8899 -73255
Department
Cellular and Molecular Medicine
Lab
Ottawa Bioinformatics Core Facility
Street address
501 Smyth Rd.
City
Ottawa
State/province
ON
ZIP/Postal code
K1H 8L6
Country
Canada
Platform ID
GPL96
Series (1)
GSE3823
Molecular Signatures Orchestrating fate of Human Hematopoietic Stem Cells Originating From Different Stages of Ontogeny
Data table header descriptions
ID_REF
VALUE
'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
5756.01
P
0.000340305
AFFX-BioB-M_at
10548.5
P
4.42873e-05
AFFX-BioB-3_at
9216.75
P
0.000169227
AFFX-BioC-5_at
12803.7
P
7.00668e-05
AFFX-BioC-3_at
11627.5
P
4.42873e-05
AFFX-BioDn-5_at
15282.3
P
4.42873e-05
AFFX-BioDn-3_at
78145.3
P
5.16732e-05
AFFX-CreX-5_at
165123
P
4.42873e-05
AFFX-CreX-3_at
230910
P
4.42873e-05
AFFX-DapX-5_at
36.5465
A
0.631562
AFFX-DapX-M_at
58.0349
A
0.760937
AFFX-DapX-3_at
38.697
A
0.953518
AFFX-LysX-5_at
148.165
A
0.116113
AFFX-LysX-M_at
284.531
A
0.51489
AFFX-LysX-3_at
14.139
A
0.724854
AFFX-PheX-5_at
32.4185
A
0.910522
AFFX-PheX-M_at
60.9033
A
0.804734
AFFX-PheX-3_at
98.7266
A
0.783476
AFFX-ThrX-5_at
58.7211
A
0.672921
AFFX-ThrX-M_at
59.4395
A
0.737173
Total number of rows: 22283 Table truncated, full table size 660 Kbytes .Supplementary file
Size
Download
File type/resource
GSM87729.CEL.gz
3.2 Mb
(ftp) (http)
CEL
GSM87729.EXP.gz
500 b
(ftp) (http)
EXP