|
| Status |
Public on Feb 19, 2015 |
| Title |
GFP_24h_A |
| Sample type |
RNA |
| |
|
| Source name |
hepatocytes transduced with SINLV.PGK.GFP
|
| Organism |
Homo sapiens |
| Characteristics |
vector: SINLV.PGK.GFP
time point: 24h
cell type: primary hepatocytes
|
| Treatment protocol |
Primary human hepatocytes were transduced with SINLV.ET.trBRAF, SINLV.PGK.GFP at MOI 10 or mock-treated (controls).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA including small RNAs from hepatocytes was isolated with the miRNeasy Mini Kit (Qiagen) at different time points
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA probes were synthesized using the GeneChip Whole Transcript Sense Target Labeling Assay Kit (Affymetrix) following the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, biotinylated cRNA probe (25 ng/uL in 100 uL hybridizationconcktail) were hybridized for 17 hr at 45C on Gene Chip Human Gene 1.0 ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 (protocol FS450_0007).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
human primary hepatocytes
|
| Data processing |
The data were analyzed with Expression Console, R and Bioconductor.
|
| |
|
| Submission date |
Feb 21, 2012 |
| Last update date |
Feb 19, 2015 |
| Contact name |
Marco Ranzani |
| E-mail(s) |
[email protected]
|
| Organization name |
HSR-TIGET
|
| Department |
TIGET
|
| Lab |
Montini
|
| Street address |
via olgettina 58
|
| City |
Milan |
| ZIP/Postal code |
20132 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE35986 |
Lentiviral vector-based insertional mutagenesis identifies new clinically relevant candidate cancer genes involved in the pathogenesis of hepatocellular carcinoma |
|
