GEO help: Mouse over screen elements for information.
Status
Public on Dec 31, 2005
Title
VFB_mutant replicate 2
Sample type
RNA
Source name
5 day old seedlings
Organism
Arabidopsis thaliana Characteristics
mutant, 10 day old seedlings, light grown
Extracted molecule
total RNA
Extraction protocol
Approximately 80 mg from 5 day old light grown VFB triple mutant seedlings were collected and ground to a fine powder under liquid Nitrogen. Samples were lysed in separate groups in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label
biotin
Label protocol
Approximately 6 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
VFB triple mutant 10 day old seedlings
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 15, 2005
Last update date
Dec 19, 2005
Contact name
Claus Schwechheimer
E-mail(s)
[email protected] Organization name
TUM
Department
Plant Systems Biology
Street address
Emil-Ramann-Str. 4
City
Freising
ZIP/Postal code
85354
Country
Germany
Platform ID
GPL198 Series (1)
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was P (P), A (A), M (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
472.9
P
0.00015
AFFX-BioB-M_at
549.7
P
0.00004
AFFX-BioB-3_at
507.8
P
0.00004
AFFX-BioC-5_at
1466
P
0.00004
AFFX-BioC-3_at
975.9
P
0.00004
AFFX-BioDn-5_at
2562.8
P
0.00004
AFFX-BioDn-3_at
4422
P
0.00004
AFFX-CreX-5_at
11935.6
P
0.00004
AFFX-CreX-3_at
15883.3
P
0.00005
AFFX-DapX-5_at
348.4
P
0.00026
AFFX-DapX-M_at
603.9
P
0.00009
AFFX-DapX-3_at
1416.8
P
0.00005
AFFX-LysX-5_at
57.3
P
0.00844
AFFX-LysX-M_at
107.9
P
0.02611
AFFX-LysX-3_at
208.7
P
0.00022
AFFX-PheX-5_at
106.7
P
0.00202
AFFX-PheX-M_at
117.1
P
0.00402
AFFX-PheX-3_at
183.3
P
0.00004
AFFX-ThrX-5_at
131.8
P
0.00097
AFFX-ThrX-M_at
231.7
P
0.00017
22810 568 Kbytes .Supplementary data files not provided
