GEO help: Mouse over screen elements for information.
Status
Public on Dec 31, 2005
Title
VFB_mutant replicate 3
Sample type
RNA
Source name
5 day old seedlings
Organism
Arabidopsis thaliana Characteristics
mutant, 10 day old seedlings, light grown
Extracted molecule
total RNA
Extraction protocol
Approximately 80 mg from 5 day old light grown No-0 wild type seedlings were collected and ground to a fine powder under liquid Nitrogen. Samples were lysed in separate groups in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label
biotin
Label protocol
Approximately 6 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
VFB triple mutant 10 day old seedlings
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 15, 2005
Last update date
Dec 19, 2005
Contact name
Claus Schwechheimer
E-mail(s)
[email protected] Organization name
TUM
Department
Plant Systems Biology
Street address
Emil-Ramann-Str. 4
City
Freising
ZIP/Postal code
85354
Country
Germany
Platform ID
GPL198 Series (1)
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was P (P), A (A), M (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
374.7
P
0.00026
AFFX-BioB-M_at
521.9
P
0.00004
AFFX-BioB-3_at
371.4
P
0.00004
AFFX-BioC-5_at
1516
P
0.00004
AFFX-BioC-3_at
1003.3
P
0.00005
AFFX-BioDn-5_at
3018.5
P
0.00004
AFFX-BioDn-3_at
4230.7
P
0.00004
AFFX-CreX-5_at
12658.3
P
0.00004
AFFX-CreX-3_at
15903
P
0.00004
AFFX-DapX-5_at
497.6
P
0.00013
AFFX-DapX-M_at
793.3
P
0.00011
AFFX-DapX-3_at
1311.8
P
0.00004
AFFX-LysX-5_at
55
P
0.00097
AFFX-LysX-M_at
92.3
M
0.05421
AFFX-LysX-3_at
189.2
P
0.00008
AFFX-PheX-5_at
73.2
P
0.00619
AFFX-PheX-M_at
126.3
P
0.00159
AFFX-PheX-3_at
204.9
P
0.00017
AFFX-ThrX-5_at
154.4
P
0.0002
AFFX-ThrX-M_at
241.4
P
0.00011
22810 568 Kbytes .Supplementary data files not provided
