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Status
Public on Apr 24, 2008
Title
csn4-1_mutant replicate 1
Sample type
RNA
Source name
7 day old seedlings
Organism
Arabidopsis thaliana
Characteristics
mutant, 7 day old seedlings, light grown
Extracted molecule
total RNA
Extraction protocol
Approximately 80 mg from 7 day old light grown csn4-1 mutant seedlings were collected and ground to a fine powder under liquid Nitrogen. Samples were lysed in separate groups in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label
biotin
Label protocol
Approximately 6 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
csn4-1 mutant 7 day old seedlings light grown
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 15, 2005
Last update date
Aug 28, 2018
Contact name
Claus Schwechheimer
E-mail(s)
[email protected]
Organization name
TUM
Department
Plant Systems Biology
Street address
Emil-Ramann-Str. 4
City
Freising
ZIP/Postal code
85354
Country
Germany
Platform ID
GPL198
Series (2)
GSE3865
CSN4-1 mutant analysis
GSE9728
COP9 signalosome (csn) mutant analysis
Relations
Reanalyzed by
GSE119083
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was P (P), A (A), M (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
1903.9
P
0.00007
AFFX-BioB-M_at
2850.6
P
0.00004
AFFX-BioB-3_at
1763.9
P
0.00004
AFFX-BioC-5_at
6032.3
P
0.00004
AFFX-BioC-3_at
4427.7
P
0.00004
AFFX-BioDn-5_at
12408.8
P
0.00004
AFFX-BioDn-3_at
19063.1
P
0.00004
AFFX-CreX-5_at
47837.8
P
0.00004
AFFX-CreX-3_at
61291.1
P
0.00004
AFFX-DapX-5_at
11849.2
P
0.00004
AFFX-DapX-M_at
13825.9
P
0.00004
AFFX-DapX-3_at
26094.4
P
0.00004
AFFX-LysX-5_at
1156.9
P
0.00004
AFFX-LysX-M_at
1809.4
P
0.00004
AFFX-LysX-3_at
4004
P
0.00005
AFFX-PheX-5_at
2364.7
P
0.00004
AFFX-PheX-M_at
3241.6
P
0.00004
AFFX-PheX-3_at
5343
P
0.00005
AFFX-ThrX-5_at
3242.9
P
0.00005
AFFX-ThrX-M_at
5035.6
P
0.00004
Total number of rows: 22810 Table truncated, full table size 571 Kbytes .Supplementary file
Size
Download
File type/resource
GSM88049.CEL.gz
1.7 Mb
(ftp) (http)
CEL
Processed data included within Sample table